alexa Evaluation of conventional castaneda and lysis centrifugation blood culture techniques for diagnosis of human brucellosis.
Infectious Diseases

Infectious Diseases

Journal of Tropical Diseases & Public Health

Author(s): Mantur BG, Mangalgi SS

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Abstract We investigated the role of the lysis centrifugation blood culture technique over the conventional Castaneda technique for the diagnosis of human brucellosis. The lysis centrifugation technique has been found to be more sensitive in both acute (20\% higher sensitivity; P < 0.00001) and chronic (40\% higher sensitivity; P = 0.087) forms of brucellosis. The major advantage of lysis centrifugation was in the mean detection time, which was only 2.4 days in acute and 2.7 days in chronic cases, with 103 out of 110 (93.6\%) and 17 out of 20 (85\%) cultures from acute and chronic brucellosis, respectively, detected before the conventional culture was positive. Our results confirmed the potential usefulness of the lysis technique in diagnosis and institution of appropriate antibiotic therapy.
This article was published in J Clin Microbiol and referenced in Journal of Tropical Diseases & Public Health

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