Author(s): Ogretmen F, Inanan BE
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Abstract BACKGROUND: The cryopreservation procedures that allow preserving sperm cells have been applied for sperm of many species. A sugar like glucose, fructose and sucrose were frequently used in cryomedia but up to the present pine honey was not used for cryopreservation of sperm cells. OBJECTIVE: The objective of present study is to investigate the effect of pine honey in various concentrations of 100, 200, 300, 400 and 500 mg/ml solutions on cryopreservation and fertilization ability of spermatozoa of common carp (Cyprinus carpio). MATERIALS AND METHODS: Totally 12.5 \% (v/v) Me2SO as a cryoprotectant and 10 \% (v/v) egg yolk added all extenders. Pine honey also compared with sugars as glucose, fructose (monosaccharide) and sucrose (disaccharide). Collected semen samples were diluted at the ratio of 1:9 with the extenders. After dilutions, the sperm motility was assessed for each group and then the diluted semen samples were cryopreserved. RESULTS: The extenders containing 300 mg ml-1 pine honey group showed both highest post thaw motility 75.3 ± 5.1 \%, motility duration (s) 47.3 ± 2.5 \% and hatching ratio 42.6 ± 4.2 \% than other cryopreserved groups (P<0.05). CONCLUSION: Using the pine honey in cryomedia is effective for cryopreservation especially about hatching success of egg fertilized by frozen-thawed sperm of common carp.
This article was published in Cryo Letters
and referenced in Journal of Fisheries & Livestock Production