alexa Expression of IL-8, TNF-alpha and IFN-gamma m-RNA in ulcerative colitis, particularly in patients with inactive phase.
General Science

General Science

Journal of Biotechnology & Biomaterials

Author(s): Masuda H, Iwai S, Tanaka T, Hayakawa S

Abstract Share this page

Abstract OBJECTIVE: The etiology of ulcerative colitis (UC) remains uncertain. It has been said that in patients with an active phase of UC the levels of the proinflammatory cytokines increased. Paying particular attention to patients with inactive UC, we investigated several cytokines' m-RNA transcripts. METHODS: In 27 patients with UC, the mucosal cytokine expression of m-RNA including IL-8, TNF-alpha, IFN-gamma and IL-6 were examined using the reverse transcription-coupled polymerase chain reaction (RT-PCR) and oligonucleotide hybridization. RESULTS: 1) The expression ratios of m-RNA of IL-8, IFN-gamma and TNF-alpha in cases with UC (92.6\%, 70.4\% and 77.8\%, respectively) were significantly higher than in normal controls (17.6\%, 0.0\% and 5.9\%, respectively) (p < 0.01). Regarding IL-8 and IFN-gamma, those in UC cases were also significantly higher than in cases with other types of colitis (p < 0.05). 2) No significant difference was noted between active and inactive cases with UC in terms of the expression ratios of m-RNA of IL-8, TNF-alpha and IFN-gamma. 3) Only the expression ratio of IL-6 m-RNA correlated with the macroscopic score according to Matts. In addition, expression of IL-6 m-RNA in the colon tissue was observed in 9 out of 10 cases with other types of colitis (90.0\%). CONCLUSION: The expression of IL-8, TNF-alpha and IFN-gamma m-RNA increased even in inactive UC, suggesting that these three cytokines play an important role in the pathogenesis of repeated inflammation of UC.
This article was published in J Clin Lab Immunol and referenced in Journal of Biotechnology & Biomaterials

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords