alexa Expression, purification and crystallization of human 3'-phosphoadenosine-5'-phosphosulfate synthetase 1.


Journal of Vaccines & Vaccination

Author(s): Harjes S, Scheidig A, Bayer P

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Abstract 3'-Phosphoadenosine-5'-phosphosulfate (PAPS) is used to incorporate sulfate into biomolecules. The human PAPS synthetase 1 catalyzes two steps leading from adenosine triphosphate (ATP) and sulfate to PAPS. The ATP sulfurylase domain catalyzes the formation of the intermediate adenosine-5'-phosphosulfate (APS). The APS kinase domain then adds a phosphate group to the 3'-ribose and releases PAPS. In this article, the recombinant expression, purification and crystallization of the full-length protein is described. In Escherichia coli the protein is only partly soluble and copurifies with GroEL. The pure protein migrates as a dimer in gel-filtration chromatography. It is moderately active, forming 25 nmol PAPS per minute per milligram. Crystals grow to 100 x 100 x 300 micro m and diffract to 1.75 A. This article was published in Acta Crystallogr D Biol Crystallogr and referenced in Journal of Vaccines & Vaccination

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