Author(s): Rykova EY, Laktionov PP, Skvortsova TE, Starikov AV, Kuznetsova NP,
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Abstract A methylation-specific polymerase chain reaction technique was used to investigate aberrant promoter methylation of RASSF1A and HIC-1 genes in circulating extracellular DNA (exDNA) from the blood of breast cancer and fibroadenoma patients. Methylated DNA could be detected in the exDNA eluted from the surface of erythrocytes and leukocytes, even in the samples where no methylated DNA could be detected in plasma. The data obtained demonstrate that cell surface bound exDNA provides a valuable source of material for early noninvasive cancer diagnostics and monitoring.
This article was published in Ann N Y Acad Sci
and referenced in Journal of Molecular Biomarkers & Diagnosis