Author(s): Gardiner C, Ferreira YJ, Dragovic RA, Redman CW, Sargent IL
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Abstract Nanoparticle tracking analysis (NTA) is a light-scattering technique that is useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has been criticised for a lack of standardisation. We propose the use of silica microspheres for the calibration of NTA measurements and describe in detail a protocol for the analysis of EVs by NTA which should minimise many of the sources of variability and imprecision associated with this technique.
This article was published in J Extracell Vesicles
and referenced in Journal of Biotechnology & Biomaterials