alexa Factors affecting the loss of carbon monoxide from stored blood samples.
Toxicology

Toxicology

Journal of Clinical Toxicology

Author(s): Chace DH, Goldbaum LR, Lappas NT

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Abstract The loss of carbon monoxide (CO) from whole blood or hemolysates has been investigated. Blood samples were exposed to the atmosphere or to a limited volume of air for various storage periods at three temperatures. The initial hemoglobin (Hb) concentration and the percent carboxyhemoglobin (\%COHb) saturation were varied in separate experiments. In addition, the effect of repeated exposure of blood to air was evaluated. The \%COHb saturation decreased from 80 to 50\% following storage of a 1-mL blood sample with 49 mL of air in a sealed container at room temperature for 45 hr. Greater decreases in the \%COHb saturation were observed in samples which were exposed to the atmosphere. Lesser, but significant, losses occurred when samples were stored in a refrigerator or freezer. The concentration of Hb in the samples as well as the initial \%COHb saturation were found to influence the decrease in the \%COHb saturation.
This article was published in J Anal Toxicol and referenced in Journal of Clinical Toxicology

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