Author(s): Safavi A, Karimi MA, Nezhad MR
Abstract Share this page
Abstract A chemiluminescent method for the determination of isoniazid is described. Method is based on the chemiluminescence (CL) generated during the oxidation of luminol by N-bromosuccinimide (NBS) and N-chlorosuccinimide (NCS) in alkaline medium. It was found that the isoniazid could greatly enhance this CL intensity when present in the luminol solution. Based on this observation, a new flow-injection CL method for the determination of isoniazid is proposed in this paper. The detection limits were 4 and 3 ng ml(-1) isoniazid for the NBS- and NCS-luminol CL systems, respectively. The relative CL intensity was linear with the isoniazid concentration in the range of 8-600 and 600-5000 ng ml(-1) for the NBS-luminol CL system, and 6-200 and 200-2000 ng ml(-1) for the NCS-luminol CL system. The results obtained for the assay of pharmaceutical preparations compared well with those obtained by the official method and demonstrated good accuracy and precision.
This article was published in J Pharm Biomed Anal
and referenced in Pharmaceutica Analytica Acta