Author(s): Prez Ruiz T, MartnezLozano C, Toms V, Sidrach de Cardona C
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Abstract Two flow-injection methods for the fluorimetric determination of penicillamine and tiopronin are proposed. The procedures are based on the oxidation of these drugs by thallium(III). In hydrochloric acid medium the fluorescence of thallium(I) formed in the oxidation of penicillamine or tiopronin is monitored using excitation and emission wavelengths of lambda ex = 227 nm and lambda em = 419 nm respectively. Linear calibration graphs were obtained between 3 x 10(-7) and 8 x 10(-6) M for penicillamine and between 8 x 10(-7) and 2 x 10(-5) M for tiopronin with sampling frequencies of 90 and 45 samples h-1 respectively. The relative standard deviations were in the ranges 0.48-0.29\% for penicillamine and 1.04-0.31\% for tiopronin. The applicability of the method to the determination of both drugs in pharmaceutical preparations was demonstrated by investigating the effect of potential interferences and by analysis of commercial preparations.
This article was published in J Pharm Biomed Anal
and referenced in Journal of Microbial & Biochemical Technology