alexa Fluorescence investigations on choline phospholipid binding and chemical unfolding of HSP-1 2, a major protein of horse seminal plasma.


International Journal of Advancements in Technology

Author(s): Kumar CS, Sivaramakrishna D, Ravi SK, Swamy MJ

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Abstract Seminal fibronectin type-II (Fn-II) proteins interact with choline phospholipids present on the sperm plasma membrane and play a crucial role in sperm capacitation. Crystal structure of phosphorylcholine (PrC) complex of PDC-109, the major bovine Fn-II protein, together with fluorescence spectroscopic studies has shown that tryptophan residues are crucial for its specific interaction with choline phospholipids. In the present study, the heterogeneity and microenvironment of tryptophan residues in HSP-1/2, a major protein of horse seminal plasma (which is homologous to PDC-109) were investigated in the native state, in the presence of PrC and phosphatidylcholines (PCs) with short (valeryl, C-5) and long (myristoyl, C-14) chains, and upon denaturation using fluorescence quenching, time-resolved fluorescence and red-edge excitation shift (REES) measurements. The results obtained show that the environment of tryptophan residues in HSP-1/2 is more heterogeneous as compared to that in PDC-109. Binding of choline containing ligands afforded a protection to the tryptophan residues with the shielding order being: PrC≤divalaroyl PC
  • DOI: 10.1016/j.jphotobiol.2016.02.025
  • This article was published in J Photochem Photobiol B and referenced in International Journal of Advancements in Technology

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