alexa Fluorescence labeling of oligosaccharides useful in the determination of molecular interactions.
Bioinformatics & Systems Biology

Bioinformatics & Systems Biology

Journal of Glycomics & Lipidomics

Author(s): Sugahara D, Amano J, Irimura T

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Abstract A simple method to label oligosaccharides with a multifunctional fluorescent group was developed. Oligosaccharides were quantitatively labeled at their reducing termini with pyrene butanoic acid hydrazide. The pyrene-labeled oligosaccharides were successfully applied to fluorescence polarization measurements and ELISA at picomole quantity, which was not previously reached by other procedures. This labeling method should prove to be useful in a variety of aspects in glycobiology.
This article was published in Anal Sci and referenced in Journal of Glycomics & Lipidomics

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