alexa Fluorimetric determination of phenothiazine derivatives by photooxidation in a flow-injection system.
Pharmaceutical Sciences

Pharmaceutical Sciences

Pharmaceutica Analytica Acta

Author(s): Laassis B, Aaron JJ, Mahedero MC

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Abstract Flow-injection analysis (FIA) was combined with photochemically induced fluorescence (PF) detection for the determination of four phenothiazine derivatives, including unsubstituted phenothiazine, thionine, Azure A and Methylene Blue. The working analytical parameters (flow-rate, injected volume, photoreactor length) were optimized. Linear calibration graphs were obtained over about two orders of magnitude, with relative standard deviation within the range 1-2.3\%. Limits of detection were between 13 and 35 ng/ml, according to the compound. The FIA-PF method was applied to the determination of phenothiazines in urine samples. Mean recoveries ranged from 94 to 117\%.
This article was published in Talanta and referenced in Pharmaceutica Analytica Acta

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