Author(s): Garca AlvarezCoque MC, Medina Hernndez MJ, Villanueva Camaas RM, Mongay Fernndez C
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Abstract o-Phthalaldehyde reacts with amino acids in the presence of a thiol to give highly fluorescent 1-alkyl-thio-2-alkyl-substituted isoindoles. However, the instability of the derivatives limits the general utility of the reaction. Mechanistic descriptions of isoindole formation and degradation proposed in the last years, which permit a better understanding of the factors affecting isoindole stability, are presented. The use of alternative thiols and o-phthalaldehyde-like reagents is also reviewed.
This article was published in Anal Biochem
and referenced in Journal of Chromatography & Separation Techniques