Author(s): Hoti SL, Balaraman K
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Abstract A mutant of Bacillus thuringiensis H-14 produced a dark brown pigment during sporulation. Production of the pigment depended on the nutritional properties of the growth medium. The pigment was identified as melanin, based on chemical tests and its infra-red spectrum. Incorporation of L-tyrosine in the culture medium enhanced the level of melanin production, and L-3,4-dihydroxyphenylalanine (L-DOPA) was detected in the culture broth during the late-exponential phase of growth. This indicates that the pathway of melanin synthesis is from L-tyrosine, via L-DOPA, to melanin.
This article was published in J Gen Microbiol
and referenced in Biochemistry & Pharmacology: Open Access