alexa Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization. Monoclonal antibody for a ginseng saponin.
Chemistry

Chemistry

Pharmaceutical Analytical Chemistry: Open Access

Author(s): Fukuda N, Tanaka H, Shoyama Y, Fukuda N, Tanaka H, Shoyama Y

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Abstract The ratio of hapten and bovine serum albumin in an antigenconjugate was determined by matrix-assisted laserdesorption/ionization time-of-flight mass spectrometry. Ahybridoma secreting monoclonal antibody against ginsenosideRg(1) was produced by fusing sprenocytes immunized with aginsenoside Rg(1)-bovine serum albumin conjugate withHAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very smallcross-reaction appeared with ginsenoside Re. The full measuringrange of the assay extends from 0.3 mug ml(-1) to 10 mug ml(-1) ofginsenoside Rg(1).
This article was published in Cytotechnology and referenced in Pharmaceutical Analytical Chemistry: Open Access

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