alexa FTIR-ATR infrared spectroscopy for the detection of ochratoxin A in dried vine fruit.
Infectious Diseases

Infectious Diseases

Journal of Infectious Diseases & Therapy

Author(s): GalvisSnchez AC, Barros A, Delgadillo I

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Abstract A method of screening sultanas for ochratoxin A (OTA) contamination, using mid-infrared spectroscopy/Golden Gate single-reflection ATR (attenuated total reflection), is described. The main spectral characteristics of sultanas from different sources were identified in a preliminary acquisition and spectral analysis study. Principal component analysis (PCA) showed that samples of various origins had different spectral characteristics, especially in water content and the fingerprint region. A lack of reproducibility was observed in the spectra acquired on different days. However, spectral repeatability was greatly improved when water activity of the sample was set at 0.62. A calibration curve of OTA was constructed in the range 10-40 microg OTA kg(-1). Samples with OTA levels higher than 20 microg kg(-1) were separated from samples contaminated with a lower concentration (10 microg OTA kg(-1)) and from uncontaminated samples. The reported methodology is a reliable and simple technique for screening dried vine fruit for OTA. This article was published in Food Addit Contam and referenced in Journal of Infectious Diseases & Therapy

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