alexa Fungal proteins with mannanase activity identified directly from a Congo Red stained zymogram by mass spectrometry.


Journal of Microbial & Biochemical Technology

Author(s): Peterson R, Grinyer J, Joss J, Khan A, Nevalainen H

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Abstract Secreted fungal proteins with mannanase activity were identified by mass spectrometry of bands excised from a Congo Red stained zymogram containing locust bean gum as substrate. This technique circumvents the need to locate corresponding bands on a parallel gel without substrate and provides good accuracy in targeting proteins for identification. This article was published in J Microbiol Methods and referenced in Journal of Microbial & Biochemical Technology

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