Author(s): TrbojevicCepe M, Kracun I, Jusic A, Pavlicek I
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Abstract Simultaneous profile determination and quantification of human cerebrospinal fluid (CSF) gangliosides in various neurologic diseases (n = 71) was examined. Gangliosides were extracted with methanol/chloroform from clinically available amounts of CSF (4-5 ml), then separated and quantified by high-performance thin-layer chromatography (HPTLC) and direct densitometry. Based on chromatographic comparison with standards, the percentage of lipid-bound NeuAc positive fractions in 'normal' CSF samples were: GM1 (II3 NeuAc-GgOse4Cer) (3\%); GD3 (II3 NeuAc2-Lac-Cer) (4\%); GD1a (IV3 NeuAc, II3 NeuAc-GgOse4 Cer) (15\%); X1 (3\%); GD1b (II3(NeuAc)2-GgOse4 Cer) (16\%); X2 (4\%); GT1b (IV3 NeuAc, II3(NeuAc)2-GgOse4-Cer) (40\%); and GQ1b (IV3(NeuAc)2, II3(NeuAc)2-GgOse4-Cer (15\%). Similarity between CSF and CSF and human cerebellar cortex, particularly in proportion of "b" series gangliosides (GQ1b, GT1b, GD1b), could be observed. A higher proportion of GD1a ganglioside, with decreased GQ1b was found in infancy. The total ganglioside content (mean +/- 2 SD) varied between 645-894 micrograms/l. Significant alterations of the CSF ganglioside profile, with an increase in less polar gangliosides, GM3 and GD3, correlated with the blood-brain barrier dysfunction (CSF hemorrhages, compressive syndrome), or some malignant processes (metastatic brain melanoma). A statistically significant increase in the content of total CSF gangliosides was found in the following groups of patients as compared to controls: (1) ischemic cerebrovascular accident (CVI) with good outcome (P less than 0.02); (2) peripheral neuropathy and polyneuropathy (P less than 0.001) and (3) intravertebral discopathy (P less than 0.05). A significant decrease in the content of total CSF gangliosides was found in CVI group with lethal outcome (P less than 0.05).
This article was published in J Neurol Sci
and referenced in Journal of Glycomics & Lipidomics