alexa GC-MS analysis of water-soluble organics in atmospheric aerosol: response surface methodology for optimizing silyl-derivatization for simultaneous analysis of carboxylic acids and sugars.
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): Pietrogrande MC, Bacco D

Abstract Share this page

Abstract This paper describes the development of a derivatization procedure - silylation using N,O-bis(trimethylsilyl)-trifluoroacetamide (BSTFA) - for the simultaneous GC-MS analysis of a wide range of water-soluble organics in atmospheric aerosols. The reaction operating conditions were optimized using the response surface methodology (RSM) including central composite design (CCD) in order to achieve the highest response for a large number of dicarboxylic acids and sugars. The factors considered were: (i) reaction temperature (50-90°C), (ii) the reaction duration (60-120 min), (iii) reagent concentrations (10-100\% of the total solution volume) and (iv) pyridine concentration (0-50\% of the derivatization reagent). On the basis of RSM and experimental evidence, the optimum derivatization conditions were defined as reaction temperature of 75°C, reaction duration of 70 min, BSTFA reagent concentration of 55\% and pyridine concentration of 35\%. The optimized protocol was extended to a broader range of 22 target analytes that are relevant chemical markers, i.e., 15 carboxylic acids and 7 sugars. In addition, the applicability of the optimized procedure was verified in environmental matrices from PM filters collected under different conditions, i.e., different seasons (summer vs. winter), different sampling sites (urban vs. rural), different particle size dimensions (PM(2.5) vs. PM(1)). Copyright © 2011 Elsevier B.V. All rights reserved. This article was published in Anal Chim Acta and referenced in Journal of Chromatography & Separation Techniques

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords