Author(s): Tan H, Wu J, Lao L, Gao C
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Abstract Poly(lactide-co-glycotide) (PLGA) microspheres integrated into gelatin/chitosan/hyaluronan scaffolds were fabricated by freeze-drying and crosslinking with 1-ethyl-3-(3-dimethyl aminopropyl)carbodiimide. The effects of the microspheres on porosity, density, compressive modulus, phosphate-buffered saline uptake ratio and weight loss of the scaffolds were evaluated. Generally, a scaffold with a higher PLGA content had a lower porosity and weight loss, and a medium uptake ratio, but a larger apparent density and compressive modulus. When the PLGA content was lower than 50\%, the PLGA-integrated scaffolds had a similar pore size (approximately 200microm) as that of the control, and as much as 90\% of their porosity could be preserved. In vitro chondrocyte culture in the 50\% PLGA-integrated scaffold demonstrated that the cells could proliferate and secrete extracellular matrix at the same level as in the control gelatin/chitosan/hyaluronan scaffold.
This article was published in Acta Biomater
and referenced in Journal of Biotechnology & Biomaterials