alexa Genistein and zinc synergistically enhance gene expression and mineralization in osteoblastic MC3T3-E1 cells.
Psychiatry

Psychiatry

Journal of Addiction Research & Therapy

Author(s): Uchiyama S, Yamaguchi M

Abstract Share this page

Abstract Genistein, a phytoestrogen, and zinc, an essential trace element, have an anabolic effect on bone components. We investigated whether the combination of genistein and zinc has additive and synergistic effects in osteoblastic cells in vitro. Osteoblastic MC3T3-E1 cells with subconfluency were cultured for 48 h in medium containing either vehicle, genistein (10(-6) or 10(-5) M), zinc sulfate (10(-5) or 10(-4) M) or genistein (10(-6) or 10(-5) M) plus zinc sulfate (10(-5) or 10(-4) M). The protein content in the osteoblastic cells was significantly increased in the presence of genistein (10(-5) M) or zinc (10(-4) M). This increase was significantly enhanced with a culture of genistein (10(-5) M) plus zinc (10(-4) M). The expression of mRNAs of alpha1(I) collagen and osteocalcin, which are bone matrix protein, in osteoblastic cells was demonstrated using reverse transcription-polymerase chain reaction (RT-PCR) analysis with a specific primer. This expression was not significantly increased with genistein (10(-6) or 10(-5) M) or zinc (10(-5) or 10(-4) M). The combination of genistein (10(-6) or 10(-5) M) and zinc (10(-5) or 10(-4) M) caused a significant increase in alpha1(I) collagen and osteocalcin mRNA expression in osteoblastic cells. Alkaline phosphatase mRNA expression in osteoblastic cells was significantly enhanced with a culture of genistein (10(-5) M) plus zinc (10(-4) M). The effect of genistein (10(-5) M) plus zinc (10(-4) M) in enhancing the expression of alpha1(I) collagen, alkaline phosphatase, or osteocalcin mRNAs in osteoblastic cells was completely prevented with a culture of cycloheximide (10(-7) M), an inhibitor of protein synthesis, or 5,6-dichloro-1-beta-D-ribofuranosylbenimidazole (DRB) (10(-6) M), an inhibitor of transcriptional activity. Moreover, a culture with genistein (10(-5) M) or zinc (10(-4) M) for 14 or 21 days caused a significant increase in mineralization. This increase was markedly enhanced with a culture of the combination of genistein (10(-5) M) and zinc (10(-4) M). This study demonstrates that the combination of genistein and zinc can synergistically enhance gene expression and mineralization in osteoblastic cells.
This article was published in Int J Mol Med and referenced in Journal of Addiction Research & Therapy

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords