alexa Genomic and coexpression analyses predict multiple genes involved in triterpene saponin biosynthesis in Medicago truncatula.
Biochemistry

Biochemistry

Journal of Plant Biochemistry & Physiology

Author(s): Naoumkina MA, Modolo LV, Huhman DV, UrbanczykWochniak E, Tang Y,

Abstract Share this page

Abstract Saponins, an important group of bioactive plant natural products, are glycosides of triterpenoid or steroidal aglycones (sapogenins). Saponins possess many biological activities, including conferring potential health benefits for humans. However, most of the steps specific for the biosynthesis of triterpene saponins remain uncharacterized at the molecular level. Here, we use comprehensive gene expression clustering analysis to identify candidate genes involved in the elaboration, hydroxylation, and glycosylation of the triterpene skeleton in the model legume Medicago truncatula. Four candidate uridine diphosphate glycosyltransferases were expressed in Escherichia coli, one of which (UGT73F3) showed specificity for multiple sapogenins and was confirmed to glucosylate hederagenin at the C28 position. Genetic loss-of-function studies in M. truncatula confirmed the in vivo function of UGT73F3 in saponin biosynthesis. This report provides a basis for future studies to define genetically the roles of multiple cytochromes P450 and glycosyltransferases in triterpene saponin biosynthesis in Medicago.
This article was published in Plant Cell and referenced in Journal of Plant Biochemistry & Physiology

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

  • 2nd International Conference on Plant Physiology & Pathology
    June 26-28, 2017 Bangkok, Thailand
  • 7th World Summit on Plant Genomics
    July 03-05, 2017 Bangkok, Thailand
  • 3rd Global Summit on Plant Science
    August 07-09, 2017 Rome, Italy
  • 2nd International Conference on Biochemistry
    Sep 21-22, 2017 Macau, Hong Kong

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords