alexa Genomics in cereals: from genome-wide conserved orthologous set (COS) sequences to candidate genes for trait dissection.


Journal of Plant Biochemistry & Physiology

Author(s): Quraishi UM, Abrouk M, Bolot S, Pont C, Throude M,

Abstract Share this page

Abstract Recent updates in comparative genomics among cereals have provided the opportunity to identify conserved orthologous set (COS) DNA sequences for cross-genome map-based cloning of candidate genes underpinning quantitative traits. New tools are described that are applicable to any cereal genome of interest, namely, alignment criterion for orthologous couples identification, as well as the Intron Spanning Marker software to automatically select intron-spanning primer pairs. In order to test the software, it was applied to the bread wheat genome, and 695 COS markers were assigned to 1,535 wheat loci (on average one marker/2.6 cM) based on 827 robust rice-wheat orthologs. Furthermore, 31 of the 695 COS markers were selected to fine map a pentosan viscosity quantitative trait loci (QTL) on wheat chromosome 7A. Among the 31 COS markers, 14 (45\%) were polymorphic between the parental lines and 12 were mapped within the QTL confidence interval with one marker every 0.6 cM defining candidate genes among the rice orthologous region. This article was published in Funct Integr Genomics and referenced in Journal of Plant Biochemistry & Physiology

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

  • 3rd Global Summit on Plant Science
    August 07-09, 2017 Rome, Italy
  • 2nd International Conference on Biochemistry
    Sep 21-22, 2017 Macau, Hong Kong

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version