alexa Genotoxicity of titanium dioxide (TiO2) nanoparticles at two trophic levels: plant and human lymphocytes.
Environmental Sciences

Environmental Sciences

Journal of Pollution Effects & Control

Author(s): Ghosh M, Bandyopadhyay M, Mukherjee A

Abstract Share this page

Abstract The environmental fate and behaviour of titanium dioxide (TiO(2)) nanoparticles is a rapidly expanding area of research. There is a paucity of information regarding toxic effect of TiO(2) nanoparticles in plants and to certain extent in humans. The present study focuses on the effect of exposure of TiO(2) nanoparticles in two trophic levels, plant and human lymphocytes. The genotoxicity of TiO(2) nanoparticles was evaluated using classical genotoxic endpoints, comet assay and DNA laddering technique. DNA damaging potential of TiO(2) nanoparticles in Allium cepa and Nicotiana tabacum as representative of plant system could be confirmed in the comet assay and DNA laddering experiments. In Allium micronuclei and chromosomal aberrations correlated with the reduction in root growth. We detected increased level of malondialdehyde (MDA) concentration at 4mM (0.9 μM) treatment dose of TiO(2) nanoparticles in Allium cepa. This indicated that lipid peroxidation could be involved as one of the mechanism leading to DNA damage. A comparative study of the cytotoxic and genotoxic potential of TiO(2) nanoparticles and bulk TiO(2) particles in human lymphocytes also reveal interesting results. While TiO(2) nanoparticles were found to be genotoxic at a low dose of 0.25 mM followed by a decrease in extent of DNA damage at higher concentrations; bulk TiO(2) particles reveal a more or less dose dependent effect, genotoxic only at dose 1.25 mM and above. The study thus confirms the genotoxic potential of TiO(2) nanoparticles in both plant and human lymphocytes. Copyright © 2010 Elsevier Ltd. All rights reserved. This article was published in Chemosphere and referenced in Journal of Pollution Effects & Control

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version