alexa Global characterization of cell-specific gene expression through fluorescence-activated sorting of nuclei.


Journal of Antivirals & Antiretrovirals

Author(s): Zhang C, Barthelson RA, Lambert GM, Galbraith DW

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Abstract We describe a simple and highly effective means for global identification of genes that are expressed within specific cell types within complex tissues. It involves transgenic expression of nuclear-targeted green fluorescent protein in a cell-type-specific manner. The fluorescent nuclei are then purified from homogenates by fluorescence-activated sorting, and the RNAs employed as targets for microarray hybridization. We demonstrate the validity of the approach through the identification of 12 genes that are selectively expressed in phloem.
This article was published in Plant Physiol and referenced in Journal of Antivirals & Antiretrovirals

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