alexa Growth kinetics and self-renewal of human mesenchymal stem cells derived from bone marrow of children with oncohematological diseases during expansion in vitro.
Microbiology

Microbiology

Journal of Antivirals & Antiretrovirals

Author(s): Isaikina Y, Kustanovich A, Svirnovski A

Abstract Share this page

Abstract AIM: We investigated the ability of mesenchymal stem cells (MSCs) derived from bone marrow (BM) of children with oncohematological diseases after chemotherapy and radiation therapy to the self-renewal and proliferation for further expansion and their implementation as co-transplant, together with autologous hemopoietic stem cells (HSCs). MATERIALS AND METHODS: BM samples from 20 patients with tumors and BM samples from healthy children-donors (for allogenic HSCs transplantation) were under study. The colony forming unit-fibroblast (CFU-F) assay and the colony forming unit-granulocytes and macrophages (CFU-GM) assay were used for the evaluation of MSCs and HSCs proliferative capacity. Mononuclear cells (MNCs) were plated 1 x 10(6). Following 14 days cells subcultured in primary culture (I passage) to study MSCs ability to self-renewal in culture. 6 passages were performed. The population doubling (PD) in each passage and the cumulative population doubling were calculated. RESULTS: Data revealed 3-4-fold reduction of the mean incidence of CFU-F in patients after treatment compared to donors. The number of CFU-GM in patients was lower than in donors as well (39.0, 9.0-130.0 vs 95.0, 31-205). Correlation between numbers of CFU-F and CFU-GM was revealed in patients BM (r = 0.63, p < 0.003) as well as in healthy children. MSCs from BM possessed high potential to self-renewal. Their number increased 900-fold in primary cultures in the first 14 days and 17-fold in subculture in 60 days. The analysis of cumulative PD in patients and donors demonstrated the slowing of cell proliferation and telomere length decrease from passage to passage. CONCLUSION: Despite the low content MSCs from BM of children with oncohematological diseases possessed high potential to self-renewal. The total number of MSCs can be increased 1.5 x 10(4) fold in 2 months period in vitro.
This article was published in Exp Oncol and referenced in Journal of Antivirals & Antiretrovirals

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

p[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords