alexa Growth of Pseudomonas chlororaphis on a polyester-polyurethane and the purification and characterization of a polyurethanase-esterase
Environmental Sciences

Environmental Sciences

Journal of Pollution Effects & Control

Author(s): Gary T Howard, Carmen Ruiz, Newton Hilliard

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A Pseudomonas chlororaphis was found to degrade and utilize apolyester polyurethane as a sole carbon and energy source. Polyurethane utilization by P.chlororaphis followed simple Michaelis–Menten kinetics. The Ks and μmax values were 0.802 mg·ml−1 and 1.316 doublings·h−1, respectively. The enzymes from P. chlororaphis responsible for polyurethanedegradation were found to be extracellular. Analysis of the polyurethane degrading proteins, usingnon-denaturing polyacrylamide gel electrophoresis, revealed three active protein bands with Rf values of 0.25, 0.417 and 0.917. A polyurethane degrading enzyme was purifiedand displayed esterase activity. This enzyme was inhibited by phenylmethylsulfonyl fluoride andhad a molecular weight of 27,000 daltons.

This article was published in International Biodeterioration & Biodegradation and referenced in Journal of Pollution Effects & Control

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