alexa Hematopoietic progenitor cells are resistant to dimethyl sulfoxide toxicity.
Haematology

Haematology

Journal of Blood Disorders & Transfusion

Author(s): Branch DR, Calderwood S, Cecutti MA, Herst R, Solh H

Abstract Share this page

Abstract BACKGROUND: A direct chemical toxicity of dimethyl sulfoxide (DMSO) to hematopoietic progenitor cells has been suggested. However, a recent study failed to corroborate these earlier findings. Thus, a series of experiments was undertaken to address this issue. STUDY DESIGN AND METHODS: Bone marrow was collected from 18 donors and cryopreserved with 10 percent (vol/vol) DMSO. Aliquots of frozen bone marrow were thawed, diluted with ACD-A to 8 percent (vol/vol) DMSO, and allowed to remain in DMSO for up to 2 hours before mononuclear cells were plated for colony-forming assays. After 14 days in culture, burst-forming units-erythroid, colony-forming units--granulocyte/macrophage, and colony-forming units--granulocyte/erythrocyte/macrophage/megakaryocyte colonies were enumerated. RESULTS: There was no significant difference (p > 0.5) seen in colony formation over the 2-hour exposure to DMSO. CONCLUSION: These results support and extend a previous study that bone marrow hematopoietic progenitor cells, including burst-forming units--erythroid, colony-forming units--granulocyte/macrophage, and colony-forming units--granulocyte/erythrocyte/macrophage/megakaryocyte are resistant to any toxic effects of 8- to 10-percent (vol/vol) DMSO during at least 2 hours of DMSO exposure.
This article was published in Transfusion and referenced in Journal of Blood Disorders & Transfusion

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords