Author(s): Salam OM, Sleem AA, Omara EA, Hassan NS
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Abstract The aim of this study was to investigate the effect of misoprostol, silymarin or the co-administration of misoprostol + silymarin on the carbon tetrachloride (CCl(4))-induced hepatic injury in rats. Misoprostol (10, 100, 1000 microg/kg), silymarin (25 mg/kg) or misoprostol (100 microg/kg) + silymarin (25 mg/kg) was given once daily orally simultaneously with CCl(4) and for 15 days thereafter. The results showed that misoprostol (10, 100 or 1000 microg/kg) conferred significant protection against the hepatotoxic actions of CCl(4) in rats, reducing serum alanine aminotransferase (ALT) levels by 24.7\%, 42.6\% and 49.4\%, respectively compared with controls. Misoprostol, given at 100 or 1000 microg/kg, decreased aspartate aminotransferase (AST) by 28 and 43.6\% and alkaline phosphatase (ALP) by 19.3\% and 53.4\% respectively. Meanwhile, silymarin reduced ALT, AST and ALP levels by 62.7\%, 66.1\% and 65.1\% respectively. The co-administration of misoprostol (100 microg/kg) and silymarin (25 mg/kg) resulted in 61.4\%, 66.1\% and 57.5\% reduction in ALT, AST and ALP levels respectively. Histopathological alterations and depletion of hepatocyte glycogen and DNA content by CCl(4) were markedly reduced after treatment with misoprostol, silymarin or misoprostol + silymarin. Image analysis of liver specimens revealed a marked reduction in liver necrosis; area of damage: 32.4\%, 24\% and 10.2\% after misoprostol (10, 100 or 1000 microg/kg), 7.2\% after silymarin and 10.9\% after treatment with misoprostol 100 microg/kg + silymarin, compared with CCl(4) control group (46.7\%). These results indicate that treatment with misoprostol protects against hepatocellular necrosis induced by CCl(4). This study suggests a potential therapeutic use for misoprostol in liver injury.
This article was published in Fundam Clin Pharmacol
and referenced in Journal of Cytology & Histology