Author(s): Friedland AE, Tzur YB, Esvelt KM, Colaicovo MP, Church GM,
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Abstract We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans germ line using single-guide RNAs that are expressed from a U6 small nuclear RNA promoter. Our results demonstrate that targeted, heritable genetic alterations can be achieved in C. elegans, providing a convenient and effective approach for generating loss-of-function mutants.
This article was published in Nat Methods
and referenced in Journal of Computer Science & Systems Biology