alexa High glucose can induce lipid peroxidation in the isolated rat glomeruli.
Biochemistry

Biochemistry

Biochemistry & Physiology: Open Access

Author(s): Ha H, Yoon SJ, Kim KH

Abstract Share this page

Abstract The present study examined the effect of elevated glucose on glomerular cell membrane lipid peroxidation. Glomeruli were isolated from male Sprague-Dawley rat kidneys utilizing a standard sieving method and incubated in medium containing different concentrations of glucose at 37 degrees C for one and 48 hours. The levels of lipid peroxides (LPO) in the glomeruli were quantitated by malondialdehyde-thiobarbituric acid adduct formation. The levels of LPO in the glomeruli were significantly increased after one hour of exposure to high glucose (HG; 30 mM) medium, and this increase was sustained after 48 hours of exposure to HG. In contrast, osmotic control media containing either L-glucose or mannitol did not increase glomerular LPO. Dimethylthiourea (20 mM), a hydroxyl radical scavenger, completely blocked the increase of LPO in the glomeruli exposed to HG not only for one hour but also for 48 hours without altering LPO levels in the glomeruli exposed to control glucose. This provides evidence that oxidative stress can be induced by high ambient concentrations of glucose. Pretreatment with PKC antagonists, either 500 microM H-7 or 100 nM staurosporine, prevented the increase of LPO in the glomeruli exposed to HG for one hour but not in glomeruli exposed for 48 hours. These data suggest that (i) elevated glucose levels per se can cause oxidative stress and augment membrane lipid peroxidation of glomeruli, and (ii) activation of PKC may play a role in early phase of glucose-induced glomerular lipid peroxidation.
This article was published in Kidney Int and referenced in Biochemistry & Physiology: Open Access

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

  • 2nd International Conference on Biochemistry
    September 28-29, 2017 Dubai, UAE

  • 3rd Annual Congress on Bioscience
    October 16-17, 2017 Dubai,UAE

  • 3rd International Conference on Transcriptomics
    October 30 - November 01, 2017 Bangkok, Thailand

  • 3rd International Conference on Genetic and Protein Engineering (10 Plenary Forums - 1 Event)
    November 08-09, 2017 Las Vegas, USA

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords