alexa High-efficiency labeling of sialylated glycoproteins on living cells.
Bioinformatics & Systems Biology

Bioinformatics & Systems Biology

Journal of Glycomics & Lipidomics

Author(s): Zeng Y, Ramya TN, Dirksen A, Dawson PE, Paulson JC

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Abstract We describe a simple method for efficiently labeling cell-surface sialic acid-containing glycans on living animal cells. The method uses mild periodate oxidation to generate an aldehyde on sialic acids, followed by aniline-catalyzed oxime ligation with a suitable tag. Aniline catalysis dramatically accelerates oxime ligation, allowing use of low concentrations of aminooxy-biotin at neutral pH to label the majority of cell-surface sialylated glycoproteins while maintaining high cell viability.
This article was published in Nat Methods and referenced in Journal of Glycomics & Lipidomics

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