alexa High-efficiency yeast transformation using the LiAc SS carrier DNA PEG method.
Environmental Sciences

Environmental Sciences

Journal of Bioremediation & Biodegradation

Author(s): Gietz RD, Schiestl RH

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Abstract Here we describe a high-efficiency version of the lithium acetate/single-stranded carrier DNA/PEG method of transformation of Saccharomyces cerevisiae. This method currently gives the highest efficiency and yield of transformants, although a faster protocol is available for small number of transformations. The procedure takes up to 1.5 h, depending on the length of heat shock, once the yeast culture has been grown. This method is useful for most transformation requirements. This article was published in Nat Protoc and referenced in Journal of Bioremediation & Biodegradation

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