alexa Highly efficient and simple methods for the preparation of peroxidase and active peroxidase-antibody conjugates for enzyme immunoassays.
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): Tijssen P, Kurstak E

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Abstract The periodate-mediated conjugation of horseradish peroxidase to antibody is one of the most popular methods to prepare conjugates for enzyme immunoassays of antigens or corresponding antibodies. A very simple method to obtain peroxidase, which is both about five times cheaper than the rather expensive commercial preparations and has a significant higher activity, is reported. Moreover, the conjugation method was critically investigated and considerably simplified. Conjugates thus obtained are about three times more active than the best obtained with the original method.
This article was published in Anal Biochem and referenced in Journal of Chromatography & Separation Techniques

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