alexa HPLC UV or bioassay: two valid methods for posaconazole quantification in human serum samples.
Chemical Engineering

Chemical Engineering

Journal of Analytical & Bioanalytical Techniques

Author(s): CendejasBueno E, Forastiero A, RodriguezTudela JL, CuencaEstrella M, GomezLopez A

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Abstract In this paper we report on the development and validation of two different methods for posaconazole quantification from serum samples, HPLC/UV and bioassay. Both methods have been validated according to international guidelines and were also applied to the analysis of 61 trough serum samples from treated patients. A good correlation between both methods was observed. The HPLC method, more laborious and expensive, was demonstrated to be more accurate, precise and faster (analytical range 0.125-16 μg/mL, accuracy between -2.48 and 3.70\% and precision between 2.77 and 5.93\%, with an analytical run time of 11 min), making it a valuable tool for reference laboratories that centralize high numbers of samples. The microbiological method, however, is simple and offers sufficient precision and accuracy (analytical range 0.125-16 μg/mL, accuracy between -8.10 and 3.77\% and a precision between 4.52 and 10.07\%), to be used to monitor posaconazole. It may be a valid alternative to chromatographic methods in clinical laboratories without specialized facilities. © 2011 The Authors. Clinical Microbiology and Infection © 2011 European Society of Clinical Microbiology and Infectious Diseases. This article was published in Clin Microbiol Infect and referenced in Journal of Analytical & Bioanalytical Techniques

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