alexa HPLC-fluorescence determination of rhein,emodin,chrysophanol in Dongle plaster


Biochemistry & Analytical Biochemistry

Author(s): HAN Yuanyuan, WANG Liyong, DU Guangling, ZHAO Yanyan

Abstract Share this page

To establish an HPLC method to determine the content of rhein,emodin,chrysophanol in Dongle plaster.Methods:Phenomenex Luna C18(250 mm×4.6 mm,5 μm)column was employed with methanol-0.1% phosphoric acid(85∶15)as mobile phase,the flow rate was 1.0 mL·min-1.Compounds was determined at λex 442 nm and λem 514 nm by fluorescence detector.Results:The contents of three components could be simultaneously determined;The calibration curves were linear in the ranges of 0.2752-27.52 μg·mL-1(r=0.9999)for rhein,0.2688-26.88 μg·mL-1(r=0.9996)for emodin,0.2560-25.60 μg·mL-1(r=0.9995)for chrysophanol;The average recoveries(n=3)of rhein,emodin,and chrysophanol were 96.3%(RSD=2.4%),97.6%(RSD=2.9%),99.0%(RSD=1.9%),respectively.Conclusion:The method is accurate with good reproducibility and strong specificity.It is suitable for determination of three compounds in Dongle plaster.

  • To read the full article Visit
  • Subscription
This article was published in Chinese Journal of Pharmaceutical Analysis and referenced in Biochemistry & Analytical Biochemistry

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version