alexa Human papillomavirus infection of the male diagnosed by Southern-blot hybridization and polymerase chain reaction: comparison between urethra samples and penile biopsy samples.
Infectious Diseases

Infectious Diseases

Journal of AIDS & Clinical Research

Author(s): Kataoka A, Claesson U, Hansson BG, Eriksson M, Lindh E, Kataoka A, Claesson U, Hansson BG, Eriksson M, Lindh E

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Abstract A group of 108 healthy men aged 18 to 23 years underwent penoscopy after application of acetic acid. In 39 (37\%) aceto-white lesions were found and a biopsy was taken. From 105 patients a urethra sample was collected with a brush. The prevalence of HPV-DNA by dot-blot and Southern-blot hybridizations (DBH/SBH) and polymerase chain reaction (PCR) as well as the correlation between results from urethra samples and penile aceto-white epithelium were examined. In urethra, HPV-DNA was detected in four of the 66 patients (6\%) with normal epithelium and in three of the 39 (8\%) with aceto-white epithelium by using DBH/SBH. By PCR the corresponding findings were eight (12\%) and 10 (26\%) HPV-DNA-positive patients, respectively. Out of the 39 biopsy samples HPV-DNA was detected in six (15\%) by DBH/SBH and in 17 (44\%) by PCR. Twenty-two (56\%) of the patients with aceto-white lesions were by PCR positive in either the urethra sample or biopsy. All samples positive by DBH/SBH were also positive by PCR except for one biopsy with HPV 35, a type not tested for by PCR. HPV 11 was the most common finding in urethra and HPV 6 and 16 in aceto-white epithelium.
This article was published in J Med Virol and referenced in Journal of AIDS & Clinical Research

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