alexa Human serum is an advantageous supplement for human dermal fibroblast expansion: clinical implications for tissue engineering of skin.
Oncology

Oncology

Journal of Cancer Science & Therapy

Author(s): Mazlyzam AL, Aminuddin BS, Saim L, Ruszymah BH

Abstract Share this page

Abstract BACKGROUND: Standard fibroblast culture medium usually contains fetal bovine serum (FBS). In theory, unknown risks of infection from bovine disease or immune reaction to foreign proteins may occur if standard culture method is used for future human tissue-engineering development. Human serum (HS) theoretically would be another choice in providing a safer approach and autologous clinically reliable cells. METHODS: Isolated human dermal fibroblasts were culture-expanded in an equal volume mixture of Ham's F12 medium and Dulbecco's Modified Eagle Medium (DMEM) supplemented with either 10\% HS or 10\% FBS from passage 0 to passage 3. Effects of 10\% HS and 10\% FBS on human fibroblast viability, growth kinetics, cell cycle analysis and gene expressions were investigated and compared. RESULTS: Generally, fibroblast viability cultured in HS supplementations was much higher compared to FBS supplementation. Fibroblast proliferations were faster in HS supplementations with shorter doubling time. Cell cycle analysis showed fibroblasts cultured with HS supplementations have higher S-phase ratio compared to FBS. Gene expression levels by quantitative reverse transcriptase-polymerose chain reaction (RT-PCR) showed cultured fibroblasts with HS supplementation maintains expression of collagen type I collagen, increased expression of type III collagen and fibronectin and reduced expression of alpha-smooth muscle actin (alpha-SMA) compared to FBS. CONCLUSIONS: Results demonstrated potential advantages of HS vs. FBS in generating larger numbers of cultured dermal fibroblasts in a shorter period of time. HS also influenced mRNA expression of type III collagen and fibronectin (upregulated) and alpha-SMA (downregulated), which are important extracellular matrix proteins in wound healing. This article was published in Arch Med Res and referenced in Journal of Cancer Science & Therapy

Relevant Expert PPTs

Relevant Speaker PPTs

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords