Author(s): Chumchalov J, Smarda J
Abstract Share this page
Abstract The activity in vitro of four types of colicins (A, E1, E3, U) against one human standard fibroblast line and against 11 human tumor-cell lines carrying defined mutations of the p53 gene was quantified by MTT (tetrazolium bromide) assay. Flow cytometry showed that the pore-forming colicins A, E1 and U affected the cell cycle of 5 of these cell lines. Colicins E3 and U did not show any distinct inhibitory effects on the cell lines, while colicins E1 and especially A inhibited the growth of all of them (with one exception concerning colicin E1). Colicin E1 inhibited the growth of the tumor lines by 17-40\% and standard fibroblasts MRC5 by 11\%. Colicin A exhibited a differentiated 16-56\% inhibition, the growth of standard fibroblasts being inhibited by 36\%. In three of the lines, colicins A and E1 increased the number of cells in the G1 phase (by 12-58\%) and in apoptosis (by 7-58\%). These results correlated with the data from sensitivity assays. Hence, the inhibitory effect of colicins on eukaryotic cells in cell-selective, colicin-specific and can be considered to be cytotoxic.
This article was published in Folia Microbiol (Praha)
and referenced in Pharmaceutica Analytica Acta