Author(s): Brown SD, White CA, Bartlett MG
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Abstract Reversed-phase chromatography is the most common means of separation for small drug molecules. However, polar drugs may suffer from poor retention and peak shape in reversed-phase high-performance liquid chromatography (RP-HPLC). Hydrophilic interaction liquid chromatography (HILIC) provides a viable alternative to RP-HPLC and is an excellent way to separate polar compounds. This paper describes a HILIC/ESI-MS/MS assay for the determination of acyclovir from rat plasma, amniotic fluid, placental tissue, and fetal tissue. The isocratic separation utilizes an underivatized silica column with an acetonitrile/formate buffer mobile phase (80:20). The method is validated over a range of 50 ng/mL to 50 micro g/mL with \% error and \% relative standard deviation of <15\% over 3 days. All samples are prepared by acetonitrile protein precipitation, which yields high recovery (>84\% for acyclovir). This assay can be applied to the pharmacokinetic study of the placental transfer of acyclovir. Copyright 2002 John Wiley & Sons, Ltd.
This article was published in Rapid Commun Mass Spectrom
and referenced in Journal of Analytical & Bioanalytical Techniques