alexa Hydrophobicity indices for amino acid residues as determined by high-performance liquid chromatography.
Bioinformatics & Systems Biology

Bioinformatics & Systems Biology

Journal of Proteomics & Bioinformatics

Author(s): Cowan R, Whittaker RG

Abstract Share this page

Abstract The retention times of compounds on reversed-phase high-performance liquid chromatography columns are determined by their overall hydrophobicity. This paper exploits this relationship to derive hydrophobicity indices for amino acid residues. Retention times of 20 Z-amino acids and their methyl, ethyl and benzyl esters were determined under standard conditions on reversed-phase high-performance liquid chromatography at pH 3.0 and pH 7.5. Retention times of derivatives of six of the amino acids were used to calculate amino acid residue hydrophobicity values relative to glycine and leucine by a computer-based iterative procedure that used splines to optimize the smoothness of fit. Using these derived curves, values for each derivative of the remaining 14 Z-amino acids were determined and averages calculated. The curves, generated independently for pH 3.0 and pH 7.5 were effectively identical and the determined hydrophobicity values (other than for charged residues) were also similar at the two pHs. The values obtained vary significantly from other published values. Comparisons with some of the more commonly used hydrophobicity/hydropathy tables are presented. The highest correlation was found with constants determined for water/octanol partitioning of N- and C-terminal protected amino acids.
This article was published in Pept Res and referenced in Journal of Proteomics & Bioinformatics

Relevant Expert PPTs

Relevant Speaker PPTs

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

pharmajournals[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords