alexa Identification of all HIV type 1 group M subtypes in Senegal, a country with low and stable seroprevalence.
Microbiology

Microbiology

Journal of Antivirals & Antiretrovirals

Author(s): ToureKane C, Montavon C, Faye MA, Gueye PM, Sow PS,

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Abstract A total of 343 HIV-1-positive samples obtained between June 1996 and March 1999 was genetically characterized in the envelope region by HMA and/or sequencing. The env subtype distribution was as follows: 290 (84.6\%) A, 22 (6.5\%) B, 16 (4.7\%) C, 8 (2.5\%) D, 1 (0.03\%) E, 1 (0.03\%) F1, 4 (1.2\%) G, and 1 (0.03\%) H. For 77 samples the p24 region from the gag gene was also sequenced, and for 9 (11.6\%) the subtypes between env and gag were different. Phylogenetic tree analysis showed the predominance of AG-IBNG-like viruses among gag and env subtype A sequences. HMA is relatively simple and requires less sophisticated technical facilities compared with sequencing, and in Senegal 323 (94.2\%) of the 343 samples could be identified by this technique. However, in the actual configuration of the assay, discrimination between the recombinant AG-IBNG-like recombinant viruses, which are predominant in Senegal, and the nonrecombinant subtype A viruses is not possible. This article was published in AIDS Res Hum Retroviruses and referenced in Journal of Antivirals & Antiretrovirals

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