alexa Identification of bacterial pathogens by laser excited fluorescence
Infectious Diseases

Infectious Diseases

Journal of Tropical Diseases & Public Health

Author(s): Coburn JT, Lytle FE, Huber DM

Abstract Share this page

A rapid, laser-based method of pathogen identification has been developed. The method relies on the extent of amino-peptidase hydrolysis of a series of nonfluorescent L-amino acid β-naphthylamides to produce the highly fluorescent β-naphthylamlne by the pathogen of interest. The luminescence background was composed of Raman and Rayleigh scatter, fluorescent impurities in the buffer, β-naphthylamine fluorescence due to substrate decomposition, and emission of the biological matrix. The blank levels have been systematically examined and reduced to levels which allow the measurement of the fluorophore in the 0.1 nM range. Thus, unambiguous identification of pathogens at the 50 000 cell/mL level has been achieved. This corresponds to 2 to 3 orders of magnitude fewer cells than has been achieved by other techniques. Identification of pathogens at this level will drastically reduce the cell growth period from 48 h to 6 h and afford a more rapid turnaround time for bacterial identification.

  • To read the full article Visit
  • Subscription
This article was published in Anal Chem and referenced in Journal of Tropical Diseases & Public Health

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords