alexa Identification of F. o. cucumerinum and F. o. luffae by RAPD-generated DNA probes.


Journal of Plant Pathology & Microbiology

Author(s): Wang PH, Lo HS, Yeh Y

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Abstract AIMS: To create a fast, sensitive and specific method for identifying Fusarium oxysporum f. sp. cucumerinum and F. o. luffae. METHODS AND RESULTS: Specific DNA bands were selected as probes from RAPD profiles of 13 formae speciales of F. oxysporum. The forma specialis-specific probe OPC18300c and OPC18520f could be used to identify F. o. cucumerinum and F. o. luffae by RAPD-PCR followed dot blot hybridization, respectively. CONCLUSIONS: A specific method for identifying F. o. cucumerinum and F. o. luffae was achieved. SIGNIFICANCE AND IMPACT OF THE STUDY: F. oxysporum formae speciales identification with a DNA probe can be relatively rapid and provides a method to identify the pathogen without host inoculation tests.
This article was published in Lett Appl Microbiol and referenced in Journal of Plant Pathology & Microbiology

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