alexa Identification of new JNK substrate using ATP pocket mutant JNK and a corresponding ATP analogue.
Infectious Diseases

Infectious Diseases

Journal of Clinical Infectious Diseases & Practice

Author(s): Habelhah H, Shah K, Huang L, Burlingame AL, Shokat KM, , Habelhah H, Shah K, Huang L, Burlingame AL, Shokat KM,

Abstract Share this page

Abstract Modification of the ATP pocket on protein kinases allows selective use of an ATP analogue that exhibits high affinity for the altered kinases. Using this approach, we altered the ATP-binding site on JNK and identified N(6)-(2-phenythyl)-ATP, a modified form of ATP that exhibits high specificity and affinity for the modified, but not the wild type form, of JNK. Using modified JNK and its ATP analogue enables the detection of novel JNK substrates. Among substrates identified using this approach is heterogeneous nuclear ribonucleoprotein K, which is involved in transcription and post-transcriptional mRNA metabolism. The newly identified substrate can be phosphorylated by JNK on amino acids 216 and 353, which contribute to heterogeneous nuclear ribonucleoprotein K mediated transcriptional activities. This article was published in J Biol Chem and referenced in Journal of Clinical Infectious Diseases & Practice

Relevant Expert PPTs

Relevant Speaker PPTs

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version