Author(s): ElNaggar NelA, Haroun SA, Oweis EA, Sherief AA
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Abstract Fungi able to degrade agriculture wastes were isolated from different soil samples, rice straw, and compost; these isolates were screened for their ability to produce β-glucosidase. The most active fungal isolate was identified as Talaromyces pinophilus strain EMOO 13-3. The Plackett-Burman design is used for identifying the significant variables that influence β-glucosidase production under solid-state fermentation. Fifteen variables were examined for their significances on the production of β-glucosidase in 20 experimental runs. Among the variables screened, moisture content, Tween 80, and (NH4)2SO4 had significant effects on β-glucosidase production with confidence levels above 90\% (p < 0.1). The optimal levels of these variables were further optimized using Box-Behnken statical design. As a result, the maximal β-glucosidase activity is 3648.519 U g(-1), which is achieved at the following fermentation conditions: substrate amount 0.5 (g/250 mL flask), NaNO3 0.5 (\%), KH2PO4 0.3 (\%), KCl 0.02 (\%), MgSO4 · 7H2O 0.01 (\%), CaCl2 0.01 (\%), yeast extract 0.07 (\%), FeSO4 · 7H2O 0.0002 (\%), Tween 80 0.02 (\%), (NH4)2SO4 0.3 (\%), pH 6.5, temperature 25°C, moisture content 1 (mL/g dry substrate), inoculum size 0.5 (mL/g dry substrate), and incubation period 5 days.
This article was published in Prep Biochem Biotechnol
and referenced in Journal of Bioprocessing & Biotechniques