alexa Image-based DNA ploidy analysis aids prediction of malignant transformation in oral lichen planus.
Diabetes & Endocrinology

Diabetes & Endocrinology

Journal of Steroids & Hormonal Science

Author(s): Sperandio M, Klinikowski MF, Brown AL, Shirlaw PJ, Challacombe SJ,

Abstract Share this page

Abstract OBJECTIVES: To investigate the potential of image-based DNA ploidy analysis to predict malignant transformation in patients with oral lichen planus (OLP). STUDY DESIGN: DNA ploidy analysis was performed on biopsy samples from 14 patients with OLP who underwent malignant transformation. As controls, 42 OLP lesions showing unusual clinical features suggesting a transformation risk and 68 samples of clinically and histologically typical OLP were included. Cases with dysplasia on initial biopsy were excluded. Eighty fibroepithelial polyps acted as methodologic controls. Epithelial nuclei were isolated from formalin-fixed paraffin embedded biopsy samples and monolayers stained with Feulgen for automated image cytometry to establish DNA content. Ploidy status was correlated to outcome using Kaplan-Meier analysis and log-rank Mantel-Cox tests. RESULTS: All controls and typical OLP were diploid and none underwent malignant transformation in mean follow-up of 14 years (10-18 years). One unusual OLP developed carcinoma and all were diploid. The 14 patients with transformation developed 21 carcinomas. In the 11 patients who had a prior biopsy, 4 were aneuploid. CONCLUSIONS: DNA ploidy analysis predicted malignant transformation in more than one third (36.4\%) of patients with OLP with a preceding biopsy (n = 11). This premalignant nature could not have been diagnosed clinically or by histologic dysplasia assessment. Copyright © 2016 Elsevier Inc. All rights reserved. This article was published in Oral Surg Oral Med Oral Pathol Oral Radiol and referenced in Journal of Steroids & Hormonal Science

Relevant Expert PPTs

Relevant Speaker PPTs

  • Xavier Guerrero-Pich
    PPT Version | PDF Version
  • Alessandro Pini
    A novel synthetic antimicrobial peptide for the cure of gram-negative infections: Mechanism of action, efficacy in vivo, toxicity, bio-distribution and resistance selection
    PPT Version | PDF Version
  • K Mulkijanyan
    Plant biopolymers from Boraginaceae family species and their synthetic derivatives: Prospective pharmacological agents
    PPT Version | PDF Version
  • Manoj Kumar Chhikara
    How promising is the development of a semi-synthetic meningococcal conjugate vaccine ?
    PDF Version
  • Jianbo Wang
    Transcriptome and small RNA gene expression changes in synthetic allohexaploids of
    PPT Version | PDF Version
  • Werner Lottermoser
    Application of a Difference Electron Nanoscope (DEN): Correlation between 3D Magnetical Structures of Synthetic Fayalite with Synchrotron and Neutron Diffraction and Mössbauer Spectroscopy
    PPT Version | PDF Version
  • Jianping Yu
    Photosynthetic Ethylene Production in a Cyanobacterium
    PDF Version
  • Murad H. Al-Salamat
    Design of small synthetic molecules that mimic IL-4 binding to IL-4Rα, which therefore promotes alternate macrophage differentiation (M2) with minimal effect on the endothelial and vascular IL-4Rα.
    PDF Version

Recommended Conferences

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version