alexa Improved methodology for isolation of Acetobacter diazotrophicus and confirmation of its endophytic habitat.
Agri and Aquaculture

Agri and Aquaculture

Advances in Crop Science and Technology

Author(s): Reis VM, Olivares FL, Dbereiner J

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Abstract Nitrogen-free, semi-solid defined medium with crystallized cane sugar (100 g/l) supplemented with cane juice (5 ml/l) was the most selective for isolating Acetobacter diazotrophicus. Surveys of A. diazotrophicus using this medium showed that >10(3) cells/g fresh wt were present at all sites in all parts of the sugar cane plant and in all trash samples examined, reaching up to 10(7)/g. Additional samples, from forage grasses and cereals and from weed species collected within the sugar cane fields, were all negative. Heat treatment (50°C for 30 min) of the sugar cane setts did not affect A. diazotrophicus numbers within the plant. Nitrogenase activity of intact soil-plant systems in pots planted with heat-treated setts did not respond to inoculation with A. diazotrophicus. The endophytic habitat of this diazotroph and its propagation within the stem cuttings was confirmed. This article was published in World J Microbiol Biotechnol and referenced in Advances in Crop Science and Technology

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