alexa Improved performances of spot multiple peptide synthesis
Immunology

Immunology

Immunome Research

Author(s): Molina F, Pau B, Gougat C, Laune D, Granier C

Abstract Share this page

We have developed a new software for the design of peptides to be prepared by the manual Spot synthesis method. It covers most of the common protocols for epitope mapping and offers flexibility for planning the experiment. We have also quantified the coupling efficiencies of three different coupling methods for the synthesis of four model decapeptides. The DIC/HOBT procedure was superior (mean coupling yield per cycle: 87% to 91%) to methods using pentafluorophenyl esters of Fmoc amino acids. All four peptides prepared by the three different coupling protocols were reactive with their cognate monoclonal antibody. Finally, using the best coupling method, we synthesized peptides of increasing length (10 to 29 residues) bearing the model epitope AcPGK at the N terminus and showed that peptides of up to 29 residues were immunoreactive.

  • To read the full article Visit
  • Subscription
This article was published in Pept Res and referenced in Immunome Research

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

clinic[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords