Author(s): Bellas E, Seiberg M, Garlick J, Kaplan DL
Abstract Share this page
Abstract Current approaches to skin equivalents often only include the epidermis and dermis. Here, a full-thickness skin equivalent is described including epidermis, dermis, and hypodermis, that could serve as an in vitro model for studying skin biology or as a platform for consumer product testing. The construct is easy to handle and is maintained for >14 d while expressing physiological morphologies of the epidermis and dermis, seen by keratin 10, collagens I and IV expression. The skin equivalent produces glycerol and leptin, markers of adipose metabolism. This work serves as a foundation for understanding a few necessary factors needed to develop a stable, functional model of full-thickness skin. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
This article was published in Macromol Biosci
and referenced in Journal of Bioengineering and Bioelectronics